《植物生理学报》 2009, 45(12): 1181-1185
通信作者:计巧灵;E-mail: wji1118@yahoo.com.cn
摘 要:
亚麻品种‘双亚五号’的胚性愈伤组织诱导最佳培养基为MB (MS无机盐加B5维生素)+1.0 mg·L-1 2,4-D; 细胞初始悬浮培养的最佳培养基为MB+0.2 mg·L-1 6-BA; 细胞继代悬浮培养的最佳培养基为改良的MB (NH4NO3 减半, KNO3 加倍)+0.02 mg·L-1 2,4-D+0.2 mg·L-1 6-BA; 适宜的蔗糖量为50 g·L-1; 适宜的继代接种量为1.0~1.5 g·(25 mL)-1; 继代间隔为5~7 d。关键词:亚麻; 胚性愈伤组织; 悬浮细胞系
收稿:2009-09-23 修定:2009-12-01
资助:国家自然科学基金(30660095)和新疆教育厅重点科研项目(XJEDU2006108)。
Corresponding author: JI Qiao-Ling; E-mail: wji1118@yahoo.com.cn
Abstract:
The optimum induction medium for the embryogenic callus of Linum usitatissimum ‘Shuangya No. 5’ was MB (MS inorganic salts supplemented with B5 vitamins)+1.0 mg·L-1 2,4-D. The optimum medium for initial culture of suspension cells was liquid MB supplemented with 0.2 mg·L-1 6-BA. The suspension cells were cultured on modified MB liquid medium NH4NO3 halved, KNO3 doubled) supplemented with 0.02 mg·L-1 2,4-D+0.2 mg·L-1 6-BA for subculturing. The suitable sucrose concentration was 50 g·L-1. The optimal inoculative densities were 1.0–1.5 g·(25 mL)-1, and the subculture period was 5–7 d.Key words: Linum usitatissimum; embryogenic callus; cell suspension lines
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